(Q) biochem

1) The standard state Gibb’s free energy (
ΔG°’) for synthesis of a particular acyl-
CoA from its fatty acid and free Coenzyme A (CoASH) is +48.0 kJ/mol. Using
this value and the information contained in Table 14-4 in your textbook, calculate
the
ΔG°’ value for each of the following reactions:
fatty acid + CoASH + ATP –>
acyl-CoA + ADP + Pi
fatty acid + CoASH + ATP –>
acyl-CoA + AMP + PPi
Now write the equation and calculate the
ΔG°’ value for the reaction as it would
occur if catalyzed by Acyl-CoA synthetase and inorganic pyrophosphatase. What
drives this reaction to occur spontaneously in the cytoplasm of cells?
2) Camels can wander around in the desert for long periods of time without drinking
water. One reason for this is their hump that is full of fatty tissue. Imagine a
camel that contains 30 pounds of triacylglycerols in its hump. How much water
could be obtained by complete beta-oxidation of the fatty acids contained in this
mass of triacylglycerol. Assume that all the fatty acids in your camel are the
saturated 16-carbon palmitate.
3) Write out the sequence of reactions for the conversion of myrisoyl-CoA to
lauroyl-CoA and Acetyl-CoA. Include the names of the enzymes and any
necessary cofactors.
4) If Malonyl-CoA is synthesized from Acetyl-CoA and
14CO2, where will the 14C
end appear in palmitic acid?

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