INSTRUCTIONS TO STUDENTS: This End-of-Direction Evaluation paper contains FIVE (5) pages (including the quilt web page). You are to comprise the next particulars for your submission: Direction Code, Title of the ECA, SUSS PI No., Your Name, and Submission Date. Late submission will be subjected to the marks deduction blueprint. Please talk over with the Student Handbook for dinky print. IMPORTANT NOTE ECA Submission Closing date: 03 November 2025, 12 noon ECA Submission Guidelines Please observe the submission instructions acknowledged below: This ECA carries 70% of the course marks and is a compulsory factor. It is to be accomplished for my piece and no longer collaboratively with various students. It be crucial to post it on time. Submission You are to post the ECA project in precisely the an identical manner as your tutormarked assignments (TMA), i.e. utilizing Canvas. Submission in any various manner fancy hardcopy or any various potential is per chance no longer popular. Digital transmission is no longer instantaneous. It is that you will be ready to judge that the community web page traffic will doubtless be in particular heavy on the in the bargain of-off date and connections to the plan can’t be assured. Therefore, you are informed to post your project the day forward of the cutoff date in tell to manufacture definite the submission is popular and in moral time. If you’ll need submitted your ECA project, the place is displayed on the pc show. You are going to most attention-grabbing web a successful project submission message if you had utilized for the email notification possibility. ECA Marks Deduction Plan Please show the next: Submission Lower-off Time – Unless otherwise informed, the in the bargain of-off time for ECA submission will be at 12:00 noon on the day of the sever-off date. All submission timings will be in step with the time recorded by Canvas. Originate up Time for Deduction – College students are given a grace length of 12 hours. Therefore calculation of unhurried submissions of ECAs will originate up at 00:00 hrs the next day (this is applicable despite the indisputable reality that it is a vacation or weekend) after the sever-off date. How the Plan Works – From 00:00 hrs the next day after the sever-off date, 10 marks will be deducted for every 24-hour block. Submissions which are topic to better than 50 marks deduction will be assigned zero trace. For examples on how the blueprint works, please talk over with Fragment 5.2 Para 1.7.3 of the Student Handbook. Any additional info, missing appendices or corrections got after the in the bargain of-off date will moreover no longer be regarded as in the grading of your ECA project. Plagiarism and Collusion Plagiarism and collusion are forms of cheating and are no longer acceptable in any create of a scholar’s work, including this ECA project. It is probably going you’ll per chance presumably well relief far from plagiarism by giving appropriate references if you exhaust some various folk’s ideas, words or photos (including diagrams). Discuss along with your total knowledge on Harvard referencing and quotation: http://www.initiate.ac.uk/libraryservices/documents/Harvard_citation_hlp.pdf It is probably going you’ll per chance presumably well relief far from collusion by ensuring that your submission is in step along with your bear particular particular person effort. The electronic submission of your ECA project will be screened via a plagiarism detecting plan. For more knowledge about plagiarism and cheating, you ought to quiet talk over with the Student Handbook. SUSS takes stance against plagiarism and collusion. Serious circumstances will customarily consequence in the coed being referred to SUSS Student Disciplinary Community. For various circumstances, most important marking penalties or expulsion from the course will be imposed. To blame Utilize of Generative AI instruments Whereas Generative AI instruments equivalent to ChatGPT can generate responses for you, it can maybe not realize the particular context of your project. This will doubtless consequence in irrelevant answers or errors which is sharp to affect negatively to your grades. If it be crucial to use these instruments, it is your responsibility to seem at and validate the generated snort and rephrase for your bear words. Take into accout the reality that ideas and info taken from various sources, including those derived from the use of Generative AI instruments equivalent to ChatGPT, ought to quiet be properly attributed. Imprint that Turnitin can detect both AI generated snort and plagiarism, and it is advisable to well presumably well be topic to the penalties outlined above. For more knowledge on the to blame use of generative AI instruments and ideas to properly cite them as a provide, talk over with SUSS Instructing and Discovering out Centre’s Academic Integrity course (https://rise.pronounce.com/fragment/GlQuywqm9MmZkxbaGig0HjjFA73k7aBr#/) Query 1a Compare DNA microarray diagnosis and quantitative RT-PCR as ways for measuring gene expression. On your solution, insist how every potential works, the form of info they make, and name ONE (1) vital limitation of every potential. (8 marks) Query 1b You are investigating how the expression of a lisp gene adjustments in response to a drug treatment. Propose an experimental potential to quantify this trade in gene expression, and level to why this means is appropriate. (7 marks) Query 2a Compare Sanger sequencing with a next-generation sequencing (NGS) potential (equivalent to Illumina or Nanopore). On your solution, level to the underlying principles of every potential, and overview their throughput and current be taught lengths. (8 marks) Query 2b Discuss TWO (2) vital limitations or challenges in inspecting excessive-throughput sequencing info. Suggest a that you will be ready to judge solution or potential to address ONE (1) of the challenges. (7 marks) Query 3a Compare the underlying principles of ideas of SDS-PAGE and size exclusion chromatography (SEC) for protein separation. Illustrate how every potential separates proteins and level to the form of info every methodology offers. (7 marks) Query 3b Accumulate a purification workflow to isolate a target protein from a fancy combination utilizing an AKTA automated chromatography plan. On your solution, account for the principle steps that it is advisable to steal to purify a target protein, including as a minimum ONE (1) chromatographic methodology. Demonstrate how that it is advisable to verify the purity of the excellent protein product. (8 marks) Query 4a Compare X-ray crystallography and nuclear magnetic resonance (NMR) spectroscopy as ways for determining protein construction. Express the belief of every potential and ONE (1) key advantage and ONE (1) key limitation of every. (8 marks) Query 4b A peculiar protein has been chanced on without a experimentally solved construction. Form a methodology to foretell the three-d construction of this protein utilizing computational instruments, and overview how legitimate the anticipated mannequin is. (7 marks) Query 5a Compare the working principles of Floor Plasmon Resonance (SPR) and Bio-Layer Interferometry (BLI) for discovering out biomolecular interactions. Appraise ONE (1) neutral appropriate thing about every potential. (6 marks) Query 5b Accumulate an experiment to resolve the binding kinetics and affinity between a protein and its ligand utilizing SPR or BLI. Your solution ought to quiet account for how the experiment will doubtless be place up and the plot in which the binding info will doubtless be interpreted. (8 marks) Query 5c Computational ideas can complement experimental interaction research. Demonstrate why a computational potential (equivalent to molecular docking) is dazzling for predicting a protein–ligand interaction, and appraise ONE (1) limitation of this means. (6 marks) Query 6a Take note of the contribution of the Human Genome Mission (HGP) to the enchancment of customized tablets. Increase your solution with ONE (1) particular instance of how genomic knowledge is outdated to tailor clinical treatment to an particular particular person. (10 marks) Query 6b Propose how genomic sequencing and diagnosis can even be utilized to trace and organize an rising viral outbreak. On your solution, discuss how viral genomic info could well presumably well bid public health decisions and appraise ONE (1) allege connected with this means. (10 marks) —– END OF ECA PAPER —–
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